Providing the operating system you are running on your virtual machine complies with the minimum requirements defined on the Ctrl Suite Tech Specs page, this will not be an issue.
Ctrl Suite's License system relies on a device's hardware ID to assign a license as part of the activation process. If using a virtual machine, ensure Ctrl Engine is unlicensed before deleting your virtual machine. If the virtual machine is deleted with a license assigned to it, ON LX's license server will assume the license is still active in an offline installation.
Ctrl Suite allows two activations per license.